游离胆固醇酶法测定试剂盒
试剂盒测定方法采用CHOD-PAP终点方法结合经典GPO Trinder酶学反应,其原理为:(1) 胆固醇酯酶分解胆固醇酯为游离胆固醇。(2) 胆固醇氧化酶将游离胆固醇氧化,产生过氧化氢。(3) 在过氧化物酶催化下生色底物转化为苯醌亚胺,光密度值与胆固醇浓度成正比。
以下仅仅列举部分使用普利莱游离胆固醇测定试剂盒(E1006)发表的SCI文章,供参考。
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