鼠抗His标签单克隆抗体 (HIS.H8)(进口分装) C1301
货号 | 中文名 | 规格 | 目录价(元) |
C1301-100 | 鼠抗His标签单克隆抗体 (HIS.H8)(进口分装) | 100μl | 680 |
C1301-250 | 鼠抗His标签单克隆抗体 (HIS.H8)(进口分装) | 250μl | 1360 |
DESCRIPTION: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
SPECIFICITY: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
ISOTYPE: IgG1
TESTED APPLICATIONS: Western Blot Immunoprecipitation Immunofluorescense
IP ELISA
APPLICATIONS: Western blot: 1:1,000-1:8,000
Immunoprecipitation or immunostaining: 1:500-1:2,000
ELISA on recombinant proteins: 1:500-1:2,000
*NOTE: Optimal working dilutions must be determined by the end user
STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.
Immunoprecipitation:
IP antibody use:5ug His Mouse IgG1 per ml Lysate,WB 1:3000
1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein
3、IP (untransfected 293+anti-His mAb+ Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-His mAb+ Protein G agarose)
6、Recombinant protein (E.coli)
Western Blot Immunofluorescense
1ug His fusion protein+ Primary antibody dilution IF analysis of 293 cells transfected with
at a His tag protein, using anti-
1、1:5,000 His-Tag Mouse mAb at a 1:2000
2、1:10, 000 dilution (blue DAPI, red anti-His)
For research use only; not for use as a diagnostic.
Important Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. No claims beyond replacement of unacceptable material or refund of purchase price shall be allowed.
蛋白研究:蛋白提取、蛋白定量、Western Blot…核酸研究:核酸提取、PCR、核酸电泳… 抗体:单克隆抗体、多克隆抗体、酶标抗体… 细胞生物学:转染试剂、生化测定、细胞培养…
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